Correlation between Bmi-1 and clinicopathological features of colorectal cancer

doi:10.3969/j.issn.2095-4344.2014.06.012

Abstract

Abstract:

BACKGROUND: Bmi-1 (B-cell-specific moloney murineleukemia virus insertion site), a colorectal cancer stem cell gene is of great significance in the regulation of telomere reverse transcriptase activity and transcriptional status as well as cell aging and carcinogenesis.

OBJECTIVE: To investigate the correlation between Bmi-1 expression and clinicopathological changes of colorectal cancer.

METHODS: The expression of Bmi-1 mRNA in carcinoma cells was detected in 50 cases of colorectal cancer using quantitative real-time PCR. The expression of Bmi-1 protein in cancer tissue was detected by hematoxylin-eosin staining and immunohistochemical staining SP method.

RESULTS AND CONCLUSION: Sex, age, and tumor size showed no different effects on the expression of Bmi-1 mRNA. The expression of Bmi-1 protein showed no significant difference in patients who were different in sex, age, tumor size and Duke staging of tumor. The expressions of Bmi-1 mRNA and protein in patients with lymph node metastasis and depth of invasion deeper than the serosa were much higher than those in patients with no lymph node metastasis and depth of invasion shallower than the serosa (P < 0.05). The expression of Bmi-1 mRNA was much higher in patients with higher Duke staging cancer (P < 0.05). The expression of Bmi-1 mRNA and protein are closely related to different pathological features of colorectal cancer, such as metastasis, infiltration, and Duke staging.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: intestine, large, diagnosis, differential, genes, stem cells, tissues

CLC Number:

R394.2

Huang Zhe. Correlation between Bmi-1 and clinicopathological features of colorectal cancer[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(6): 894-899.

Figures/Tables 2

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